Russian Federation
UDC 57.085.23
UDC 582.475.2
UDC 582.475.4
Larix sibirica and Pinus sibirica explants were introduced into in vitro culture. It was found that the technology for obtaining embryogenic tissue and somatic embryogenesis differs between these species. In Larix sibirica, embryogenic potential is exhibited by zygotic embryos at the pre-cotyledonary and later developmental stages when placed on AI medium supplemented with growth regulators 2,4-D and 6-BAP at a concentration ratio of 2:1 mg/L. This leads to the formation of an embryonal-suspensor mass, successful maturation (on AI medium supplemented with ABA), germination of somatic embryos, and generation of clonal trees. In Pinus sibirica, embryogenic tissue was formed using megagametophytes containing embryos at the cleavage and post-cleavage stages, cultured on DCR and ½LV media. Improvement of protocols for somatic embryo production and selection of genetically stable cell lines is a promising direction for creating high-yielding forest plantations resistant to insect pests.
Larix sibirica, Pinus sibirica, somatic embryogenesis, clones
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