Russian Federation
The CSY3 gene, encoding the cytosolic enzyme ATP-citralase (ATP-CL, EC 4.1.3.8), contains a CpG island in its promoter, which may mediate regulation through changes in the methylation level of these islands. Primers for bisulfite sequencing were developed based on the analysis of the nucleotide sequence of the CSY3 gene promoter. The role of the methylation status of individual CG dinucleotides in the CSY3 ATP-citrate lyase gene promoter in regulating their transcript levels is demonstrated, as indicated by a direct correlation between the methylation status of the studied gene promoters and their mRNA content in maize leaf cells irradiated with light of different wavelengths. Studies show that changes in the methylation status of the CSY3 ATP-citrate lyase gene promoter in maize leaves irradiated with light of different wavelengths are associated with a redistribution of the cytosine methylation status between symmetric (CG and CNG) sites and within the asymmetric CNN site. At asymmetric sites, however, changes in the methylation status of cytosine in the presence of active phytochrome in the cell were insignificant. The phytochrome system, in particular phytochrome B, exhibits a regulatory effect at the level of controlling the expression of the CSY3 ATP-citrate lyase gene, in which a key role is played by changing the methyl status of the promoter region.
maize, DNA methylation, promoter, regulation, phytochrome
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