Russian Federation
Voronezh, Russian Federation
from 01.01.2018 to 01.01.2025 Russian Federation
As a result of laboratory studies, the efficiency of applying standard and modified clonal micropropagation protocols for three selected poplar genotypes was assessed. Conditions were optimized at all stages: sterilization, culture initiation (MS medium), multiplication (WPM medium with 0.3 mg/L 6-BAP), rooting (½ MS with IBA), and ex vitro adaptation in a peat:vermiculite substrate (3:1). The influence of genotype on sterilization efficiency was evaluated: explant contamination rates varied from 30 % to 60 % among different cultivars. The highest multiplication coefficient (5.2) and the lowest shoot height (12.8 mm) were found in the hybrid P. alba × P. tremula, while P. 'Bashkirsky' exhibited the maximum shoot height (20.8 mm) and the lowest multiplication rate (2.8). The rooting frequency of regenerants across different poplar genotypes was relatively high, ranging from 70 % to 95 %.
poplar, clonal micropropagation, in vitro culture, genotype, multiplication, rooting, ex vitro adaptation, culture medium
1. Hayrullina, M. D. Razmnozhenie topolya sverdlovskogo piramidal'nogo serebristogo selekcii N. A. Konovalova / M. D. Hayrullina, Yu. V. Plotnikova, S. N. Luganskaya // Mezhdunarodnyy zhurnal gumanitarnyh i estestvennyh nauk. – 2025. – № 1-2 (100). – S. 115–120. DOI: https://doi.org/10.36038/0131-6184-2024-5-115-120
2. Klimov, A. V. Morfologiya Populus h sibirica G. V. Krylov & G. V. Grig. ex A. K. Skvortsov v Vostochnoy Sibiri / A. V. Klimov, B. V. Proshkin // Social'no-ekologicheskie tehnologii. – 2022. – T. 12. – № 3. – S. 256–274. DOI: https://doi.org/10.31862/2500-2961-2022-12-3-284-301
3. Sivolapov, A. I. Biotehnologii i tradicionnye tehnologii razmnozheniya i sozdaniya kul'tur topolya v lesnom hozyaystve CChR / A. I. Sivolapov, V. A. Sivolapov // Aktual'naya biotehnologiya. – 2020. – № 3 (34). – S. 161–165. EDN: https://elibrary.ru/XBAZIC
4. Pavlichenko, V. V. Osobennosti mikroklonal'nogo razmnozheniya Populus ×berolinensis / V. V. Pavlichenko, M. V. Protopopova, V. K. Voynikov // Mehanizmy ustoychivosti rasteniy i mikroorganizmov k neblagopriyatnym usloviyam sredy : sbornik materialov Godichnogo sobraniya Obschestva fiziologov rasteniy Rossii, Vserossiyskoy nauchnoy konferencii s mezhdunarodnym uchastiem i shkoly molodyh uchenyh, Irkutsk, 10–15 iyulya 2018 g. : v 2 ch. – Irkutsk : Institut geografii im. V. B. Sochavy SO RAN, 2018. – Ch. II. – S. 1330–1333. DOI: https://doi.org/10.31255/978-5-94797-319-8-1330-1333; EDN: https://elibrary.ru/YAQHAD
5. Shabanova, E. A. Klonal'noe mikrorazmnozhenie hozyaystvenno-cennyh form topolya / E. A. Shabanova, O. S. Mashkina // Lesohozyaystvennaya informaciya. – 2015. – № 4. EDN: https://elibrary.ru/VADPNH
6. Sahoo Y. Micropropagation of Vitex negundo L., a woody aromatic medicinal shrub, through high-frequency axillary shoot proliferation / Y. Sahoo, P. Chand // Plant Cell Reports. – 1998. – Vol. 18. – P. 301–307. DOI: https://doi.org/10.1007/s002990050576; EDN: https://elibrary.ru/NTWWFC
7. Demenko, V. I. Osobennosti proliferacii bokovyh pobegov pri mikroklonal'nom razmnozhenii sadovyh rasteniy / V. I. Demenko, I. A. Pozdnyakov // Izvestiya Timiryazevskoy sel'skohozyaystvennoy akademii. – 2007. – № 1. – S. 47–53. EDN: https://elibrary.ru/HZDUUJ
8. Tashmatova, L. V. Vliyanie citokininov na proliferaciyu sortov yabloni v kul'ture in vitro / L. V. Tashmatova, T. M. Hromova, O. V. Macneva // Agrarnyy vestnik Urala. – 2025. – № 5. DOI: https://doi.org/10.32417/1997-4868-2025-25-05-769-778; EDN: https://elibrary.ru/XHYBQM
9. Erst, A. A. Klonal'noe mikrorazmnozhenie topolya Sibirskogo serebristogo / A. A. Erst, V. T. Bakulin // Turczaninowia. – 2012. – T. 15, № 1. – S. 108–112. EDN: https://elibrary.ru/PBHEIJ
10. Demenko, V. I. Adaptaciya rasteniy, poluchennyh in vitro, k nesteril'nym usloviyam / V. I. Demenko, V. G. Lebedev // Izvestiya Timiryazevskoy sel'skohozyaystvennoy akademii. – 2011. – № 1. – S. 52–60. EDN: https://elibrary.ru/OCBXTZ
11. Karimova, V. K. Podbor usloviy kul'tivirovaniya dlya vvedeniya in vitro topolya serebristogo (Populus alba L.) i topolya Bolle (Populus bolleana L.) / V. K. Karimova, G. K. Magzumova, A. A. Kakimzhanova // Biotehnologiya. Teoriya i praktika. – 2015. – № 1. – S. 54–63. EDN: https://elibrary.ru/VPUDAF
